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Gas Chromatography coupled to Mass Spectrometry (GC-MS)
Analytical technique dedicated to the separation, identification and quantification of mixtures of volatile and semi-volatile substances.
Separation, comparison and identification
The separation is a function of the different distribution of the substances studied between the mobile and stationary phases that make up the system.
Once separated, they are fragmented and analyzed according to their fragmentation pattern, which can be compared with information contained in a database of mass spectra for their preliminary identification.
The definitive identification, as well as the quantification of each substance must be made through the use of reference substance.
Liquid Chromatography Coupled to Mass Spectrometry (LC-MS)
Liquid chromatography coupled to mass spectrometry (LC-MS) is an analytical technique that combines the ability of separation of several organic compounds by liquid chromatography with the ability to selectively detect and confirm the molecular identity of the mass spectrometer corresponding to each separate compound. It is used for the detection and characterization of polar and semi-polar organic compounds and is not limited by the volatility or thermal stability of the analytes, as a result can be analyzed a large number of chemical species with high sensitivity.
Initiation to Solid Phase Microextraction (SPME with GC-MS)
Technique for the extraction and concentration of VOCs and SVOCs, widely used in combination with GC-MS, very useful in the food, flavoring and beverage industries.
Microextraction and desorption
SPME is based on the extraction of the analytes from the sample matrix by means of a fused silica fiber which is coated with a sorbent, in most cases polymeric, followed by the desorption of the analytes by means of temperature or an organic solvent.
As far as the desorption step is concerned, this is done either thermally or by the addition of an organic solvent, depending on the analytical technique to be used below. Thus, if the SPME is coupled to the GC, the desorption is performed thermally..